shuttle vector वाक्य
उदाहरण वाक्य
मोबाइल
- One of the most common types of shuttle vectors is the yeast shuttle vector.
- One of the most common types of shuttle vectors is the yeast shuttle vector.
- Almost all commonly used " S . cerevisiae " vectors are shuttle vectors.
- There are also adenovirus shuttle vectors, which can propagate in " E . coli " and mammals.
- Shuttle vectors are frequently used to quickly make multiple copies of the gene in " E . coli " ( amplification ).
- Yeast shuttle vectors have components that allow for replication and selection in both " E . coli " cells and yeast cells.
- However, vectors may also have elements that allow them to be maintained in another organism such as yeast, plant or mammalian cells, and these vectors are called shuttle vectors.
- The yeast component of a yeast shuttle vector includes an autonomously replicating sequence ( ARS ), a yeast centromere ( CEN ), and a yeast selectable marker ( e . g.
- The " E . coli " component of a yeast shuttle vector includes an origin of replication and a selectable marker, e . g . antibiotic resistance, beta lactamase, beta glastosidase.
- Alternatively, if replication of the DNA in different species is desired ( for example, transfer of DNA from bacteria to plants ), then a multiple host range vector ( also termed shuttle vector ) may be selected.
- Most expression vectors for extrachromosomal protein expression and shuttle vectors contain the pBR322 origin of replication, and fragments of pBR322 are very popular in the construction of intraspecies shuttle or binary vectors and vectors for targeted integration and excision of DNA from chromosome.
- Shuttle vectors include plasmids that can propagate in eukaryotes and prokaryotes ( e . g . both " Saccharomyces cerevisiae " and " Escherichia coli " ) or in different species of bacteria ( e . g . both " E . coli " and " Rhodococcus erythropolis " ).
- An expression vector has features that any Escherichia coli ", and vectors used for protein production in organisms other than " E . coli " may have, in addition to a suitable origin of replication for its propagation in " E . coli ", elements that allow them to be maintained in another organism, and these vectors are called shuttle vectors.
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